Determination of dissociation constants and specific rate constants of enzyme-substrate (or protein-ligand) interactions from rapid reaction kinetic data

Determination of dissociation constants and specific rate constants of enzyme-substrate (or protein-ligand) interactions from rapid reaction kinetic data.

The increasing availability of instruments capable of following absorbance or fluorescence changes in the millisecond time range has led in recent years to a large number of studies of ligandprotein interactions. Of particular interest to the enzymologist are cases where partial reactions in the catalytic cycle may be followed. For example, the reaction of a dehydrogenase with reduced pyridine ...

Determination of Dissociation Constants and Specific Rate Constants of Enzyme-Substrate (or Protein-Ligand) Interactions from Rapid Reaction Kinetic Data*

The increasing availability of instruments capable of following absorbance or fluorescence changes in the millisecond time range has led in recent years to a large number of studies of ligandprotein interactions. Of particular interest to the enzymologist are cases where partial reactions in the catalytic cycle may be followed. For example, the reaction of a dehydrogenase with reduced pyridine ...

NMR methods for the determination of protein-ligand dissociation constants.

This article is a review with 83 references of the application of NMR to the measurement of the dissociation constants of protein-ligand complexes. After briefly discussing some general concepts of molecular stability, the text turns to consider which NMR parameters are reporters of complex formation. The available data treatments required to translate observed NMR effects into quantitative mea...

First Principles Determination of Dissociation Rate Constants

Cyclic voltammetric simulation for electrochemically mediated enzyme reaction and determination of enzyme kinetic constants.

A cyclic voltammetric simulation that can be applied to an electrochemically mediated enzyme reaction involving any substrate and mediator concentrations was developed. Concentration polarization of the substrate in the vicinity of an electrode was considered as well as mediator concentration. Reversible and quasi-reversible electrochemical reactions with one electron followed by an enzyme reac...